分子生物学
The Center provides essential training for all instruments and technical support for troubleshooting. We also provide consultation, hands-on service on experimental design and data analysis. For more info regarding services and rates contact our Core Facility Supervisor Dr. 于静.
MAGPIX分析仪
MAGPIX分析仪, 由Luminex制造, 设备是否使用xMAP技术, 它包含了磁珠, fluorescent labeling and CCD imaging techniques, to conduct multiplex quantitative and qualitative analysis of protein or nucleic acid molecules. It is capable of examining up to 50 analytes with the sensitivity of ~106 copies of DNA or single-digit pictogram levels of protein in up to a one-hour reading period of time. Users need to purchase the assay kits which are commercially available on a wide range.
资源
http://www.bio-rad.com/en-us/product/bio-plex-magpix-multiplex-reader?ID=M3VE8D15
rt - pcr
The real-time PCR (rt - pcr) or quantitative PCR (qPCR) machine, manufactured by BioRad and modeled as CFX Connect, offers an easy-to-grab tool for amplification and quantitative analysis of DNA and/or RNA. It works with a 96-well plate platform with excitation and emission wavelength of 450-580 nm. Reaction volume ranges at 1-50 ul and it detects up to 2 targets per reaction with the sensitivity up to 1 copy of DNA.
资源
超离心机
的超离心机, manufactured by Beckman Coulter and modeled as Beckman Coulter Optima L-90K offers refrigerated high performance centrifugation for separation of small particles such as vesicles and viral particles. There are two rotors available in our Center, the SW 40 Ti wing rotor providing up to 290,000 g离心力 and the Type 70 Ti angle fixed rotor providing up to 500,000 g离心力. Special tubes are required for ultracentrifugation.
资源
http://sbmsaiumu.files.wordpress.com/2012/04/type-70-ti.pdf
http://btiscience.org/wp-content/uploads/2014/10/SW-41-Ti.pdf
凝胶和印迹成像仪
凝胶成像系统, manufactured by BioRad and modeled as ChemiDoc MP, offers a wide range of imaging from UV to near infrared light (NIF) on gels or blots for DNA and protein identification, 量化和文件化. It is capable of conducting colorimetric, fluorescent as well as chemiluminescent detections for up to three colors at a time. With RioRad stain-free gel, normalization can be done without running house-keeping genes.
资源
http://www.bio-rad.com/en-us/product/chemidoc-mp-imaging-system?ID=NINJ8ZE8Z
板的读者
The 板的读者 manufactured by Molecular Devices and modeled SpectraMax i3, is a popular tool for quantitative or qualitative detection of molecular analytes such as proteins, nucleic acids via light beam assisted immune- or biochemical reactions. Our machine holds three light detection modes, 吸光度, fluorescence and luminescence in one unit, and is able to read from both sides of a plate of 6-1536 wells for a spectrum ranging from 230-1000 nm at the user's choice. There are also multiple types of reading options such as kinetics, well scan and spectrum in addition to most commonly used endpoints.
资源
海马xfhs迷你分析仪
The Agilent 海马xfhs迷你分析仪 with the XF HS Miniplate delivers the highest sensitivity and best in Seahorse XF data quality, 工作流, and user experience - all while using less sample. The analyzer measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells in an eight-well miniplate format.
资源
Jess - capillary Western Blotting (Proteinsimple)
The Jess instrument manufactured by ProteinSimple, Bio-Techne品牌, is an instrument for faster and easier multiplexing Western blotting. It automates capillary-based protein separation and detection, ultimately shortening the Western blotting process from 1-2 days to 3 hours. Reagents and samples are loaded into microplates and proteins are separated by size using capillaries. There are 13- and 25- capillary formats to perform up to 12- and 24- blots, respectively along with the protein ladder. Detection with chemiluminescence, fluorescence or both can be done on one plate in one run. Reagents, plates and capillaries are at user's cost or purchase.
资源
http://www.proteinsimple.com/jess.html?gclid=EAIaIQobChMIo4uaoqe1-AIVhq7ICh0_egw2EAAYASABEgKUj_D_BwE